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Centrifugation, the pellets were washed three times, centrifuged, and resuspended in 200 l of buffer containing a 1 mM concentration of the substrate. After 60 min, the samples were centrifuged, and the absorbance of the supernatants was measured at 405 nm in an enzyme-linked immunosorbent assay (ELISA) reader. Control values (bacteria incubated in buffer) were used as a blank. No endogenous prote
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Ska), extracellular protein factor ( epf), or protein F2 ( prtF2) (B) were tested for surface PK/FXIIa activity after incubation with normal human plasma. (C) The PK/FXIIa activity at the surfaces of M49 wild-type and ska mutant strains was examined after exposure to normal or plasminogen-deficient plasma. Kaolin served as a positive control for plasmin-independent contact system activation. (D) P
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SENNA Monte Carlo, 1988, qualifying...what took place is that we had competition tyres, never qualifying tyres therefore it is lap immediately after lap, not only an lap. We had that turbo vehicle. We sought out, experienced a good lap, an additional lap. I became on pole, then the following lap among a larger margin, and I also was going progressively and much more and much more.i eventually got to that stage once I is over a couple seconds quicker than anyone, like our team-mate who had been using the exact same car, same motor, everything. That has been their direct comparison furthermore o
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verSenna blurs thru Eau Rouge or over that the mountain in order to Les Combes 'the chance inference to getting it mistaken and having a large shunt, as well as the chances inference of accomplishing that it ideal and exactly how a great deal you will gain.'two moments. It Absolutely Wasn't due he w
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Lyzed its impact on the modifications of histones bound at the MAGE-A1 promoters. To investigate the changes in the histone signature of MAGE-A1 promoter, it was compared in basal MCF-7 cells (no expression of MAGE-A1, Table 1) to the signature in MCF-7 cells stimulated by 5-aza-CdR with/without TSA or transfected with the expression plasmid encoding for BORIS. For these analyses we used antibodie
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Ansfected cells from untransfected cells by FACS analyses.MCF-p=0.p=0.0001 p=0.0001 p=0.Figure 4 Histone signature at the MAGE-A1 promoter as examined by chromatin immunoprecipitation. DNA was derived from unstimulated (basal) MCF-7 cells, 5-aza-CdR- and/or TSA-stimulated MCF-7 cells and MCF-7 cells transfected with the expression plasmid encoding for BORIS. DNA-bound histones were immunoprecipita
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Subsequent 48?2 h [1]. The prevalence of HAE is unclear; however, it is estimated to affect 1 in 50,000?00,000 individuals globally [4?]. The onset of symptoms typically begins during childhood; symptoms often worsen during puberty, and HAE attacksgenerally continue throughout the life of the patient [1]. HAE episodes usually occur without a predictable trigger; however, attacks can be precipitate
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Dies have confirmed this role for JCV TAg as well. Regulation of SV40 DNA replication involves the incompletely understood coordination of TAg phosphorylation and dephosphorylation. Phosphorylation of serines 120 and 123 negatively regulates TAg functions, whereas phosphorylation of threonine 124 is required for TAg-mediated replication [reviewed in 20,21]. Modifications to the corresponding resid
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E positively associated with tumor grade and ESR1, PGR, and ESR2 steroid hormone receptor expression level and negatively associated with invasive epithelial tumor cell content and tumor size. In addition, ESR2 was more highly expressed in tumors with a higher percentage of stromal cells (786 tumors with 30 to 70 invasive epithelial cells), and ESR1 wasSieuwerts et al. Breast Cancer Research 201
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