Ype and its ska mutant were incubated in normal or plasminogen-deficient plasma for 30 min. After incubation, unbound plasma proteins were removed and PK/FXIIa activity determined. Kaolin, a potent contact system activator, was used as a positive control (Fig. 1C). After incubation of M49 wild-type bacteria in plasminogen-deficient plasma, no PK/FXIIa activity was detectable (A405 0.11) compared t
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